I built my floor system on 24" centers. I used custom built 4x2 open web floor trusses (3-1/2" wide top and bottom flanges) and decked it with 1-1/8" Advantech sub-flooring. Seems pretty solid to me. My floor trusses are 32' long with the longest unsupported span being 18'6". The advantage to this approach is being able to run all of my utilities and HVAC through the trusses. This floor is above a finished basement.
